The Simpsons' farming montage music is the theme song from Green Acres.
The Southern colonel is based off the character Senator Claghorn of The Fred Allen Show. His white outfit, the pocket watch and cigars in his breast pocket resemble Claghorn's, and when he shows up at the Simpsons' house to duel, he says "Sir, I say sir!" much like Claghorn would. He also has a similar accent. Claghorn's voice and mannerisms also inspired the Looney Tunes character Foghorn Leghorn.
The Southern colonel's RV has mudflaps of himself drawn like Yosemite Sam.
The scene with Homer worrying about the duel the next morning while looking at a tombstone with his own name on it spoofs the 1990 film Back to the Future Part III.
The death of the Laramie team in the helicopter, at the hands of a Tomacco-crazed sheep, is a nod to a scene in the James Cameron film Aliens, where the crew of the marines' drop-ship meet the same fate when an alien sneaks aboard their ship.
Plutonium is shown as a glowing green liquid, even though it is a dull silver metal with a melting point of 1183 degrees Fahrenheit. However, radioactive materials are often depicted as glowing green in popular culture.
The scene where Homer thinks he is catching some invisible so-called plants was one of the idents on Channel 4 when the Simpsons moved from BBC Two to Channel 4 in the UK; the difference is that Homer thinks he is catching the Channel 4 logo surrounded by a flash of colored lights. See here from 0:30 to 0:50.
In the mid Season 20-present opening, one of the items Marge buys is a bottle of Tomacco juice.
Running Gag: Homer's tractor rolling over and crushing his legs.
When the Simpsons sneak Homer out of the house with the Christmas tree, the tree is gray, but when they stop at Donny's Discount Gas, the Christmas tree is now green, and when they resume driving the tree is gray again.
The store owner said that Abe abandoned the farm because nothing would grow, but in "Grampa vs. Sexual Inadequacy", it was said that the bank foreclosed the farm because the cows gave sour milk due to Homer scaring them.
When Lisa is criticizing the idea of the tomacco, she lifts her head up while her head's previous position stays on-screen as well. This makes it look like she has two heads at once.
When Bart is eating the tomacco, Marge says "It's very nice to see Bart eating his vegetables", even though tomatoes are fruits.