The Simpsons' farming montage music is the theme song from Green Acres.
The Southern colonel is based off the character Senator Claghorn of The Fred Allen Show. His white outfit, the pocket watch and cigars in his breast pocket resemble Claghorn's, and when he shows up at the Simpsons' house to duel, he says "Sir, I say sir!" much like Claghorn would. He also has a similar accent. Claghorn's voice and mannerisms also inspired the Looney Tunes character Foghorn Leghorn.
The Southern colonel's RV has mudflaps of himself drawn like Yosemite Sam.
The scene with Homer worrying about the duel the next morning while looking at a tombstone with his own name on it spoofs the 1990 film Back to the Future Part III.
The death of the Laramie team in the helicopter, at the hands of a Tomacco-crazed sheep, is a nod to a scene in the James Cameron film Aliens, where the crew of the marines' drop-ship meet the same fate when an alien sneaks aboard their ship.
The scene with Lenny and Carl around the water cooler is a nod to Dilbert.
Inspired by the episode, Rob Baur of Lake Oswego, Oregon actually created Tomacco; both plants are members of the Nightshade family and are therefore similar enough to create a hybrid. Baur wanted to know if it was a true hybrid, so he asked a forensic researcher to test the plant's constitution. The results showed the leaves did indeed contain nicotine. He only had one Tomacco fruit so they didn't get the chance to test it though Roy Grimsbo, the forensic scientist who performed the test for free and said he hopes Baur will bring back an actual tomacco fruit for testing.
The characters and actors listed in the credits of the Zorro movie are:
Plutonium is shown as a glowing green liquid, even though it is a dull silver metal with a melting point of 1183 degrees Fahrenheit. However, radioactive materials are often depicted as glowing green in popular culture.
The scene where Homer thinks he is catching some invisible so-called plants was one of the idents on Channel 4 when the Simpsons moved from BBC Two to Channel 4 in the UK; the difference is that Homer thinks he is catching the Channel 4 logo surrounded by a flash of colored lights. See here from 0:30 to 0:50.
The Laramie helicopter crashed but they must have saved the Tomacco plant because it is in the mid Season 20-present High Definition openings, one of the items Marge buys is a bottle of Tomacco juice.
Running Gag: Homer's tractor rolling over and crushing his legs.
When the Simpsons sneak Homer out of the house with the Christmas tree, the tree is gray, but when they stop at Donny's Discount Gas, the Christmas tree is now green, and when they resume driving the tree is gray again.
The store owner said that Abe abandoned the farm because nothing would grow, but in "Grampa vs. Sexual Inadequacy", it was said that the bank foreclosed the farm because the cows gave sour milk due to Homer scaring them(This could have been one of Grampa's crazy stories or him trying to guilt trip Homer).
When Lisa is criticizing the idea of the tomacco, she lifts her head up while her head's previous position stays on-screen as well. This makes it look like she has two heads at once.
When Bart is eating the tomacco, Marge says "It's very nice to see Bart eating his vegetables", even though tomatoes are fruits.