The movie's plot revolves around Chuck and Larry posing as a gay couple for financial reasons, similar to Moe's adopting a gay image to enhance his business.
Like Smithers demanding that Moe kiss him in public to prove that he's gay, Chuck and Larry are asked to kiss in public (at a city council hearing) to prove that their relationship is real. Chuck and Larry's kiss is stopped short, however, when their boss arrives, "outs" them as being straight and exposes their scheme.
The picture hiding the safe where Smithers gets Burns' last will from is A Bar at the Folies-Bergère by French painter Édouard Manet, only with Burns' head replacing the woman's one.
The part where Juniper is sitting on a mountain with her students and a guitar is a spoof of the part of the song Do-Re-Mi from the movie The Sound Of Music where Maria does the same thing.
The title is similar to the third season episode "Flaming Moe's," with "flaming" being a somewhat derogatory term to descrive an overtly gay man.
Renaming the bar from "Moe's" to simply "Mo's" is a reference to the slang term "mo" (short for "homo") which is an affectionate term gay men sometimes use among themselves.
Despite the premise of the epiosde, the word "gay" is only said seven times. Most references to homosexuality are done euphemistically, probably to limit the amount of "overt sexual content" in the episode, which can impact the show's parental guideline ratings.
This is one of several episodes where Maggie does not appear at all.
Mr. Burns' full name is stated to be "Charles Montgomery Plantagenet Schicklgruber Burns". Schicklgruber is the surname of Adolf Hitler's paternal grandmother Maria Anna Schicklgruber
Homer, Lenny, and Carl and their late-shift counterparts (whom they fight) bear strong resemblances to each other, with only a few differences: Carl's counterpart wears glasses, Homer's has hair on the back of his head, and Lenny's has a different hair color and style.
Moe shows pictures of his bar after previous "disasterous" makeovers:
Whiskey A Moe-Moe (A makeover Moe never actually did, but a dream he painted)
Despite what Moe claims, only two of these makeovers were failures. The Nag and Weasel was a huge success.
Moe previously had the idea to give the bar a gay makeover in "Days of Wine and D'oh'ses". When Barney announced he was quitting drinking, Moe said, "to stay afloat, this bar's gonna have to go queer."
Several one-time gay characters from previous episodes reappear, including:
Karl (Homer's executive assistant after his promotion)
Dewey Largo, who has previously been hinted as being gay and closeted, reveals his sexuality in this episode as he runs off with his partner.
Smithers says "Hello, Moe." in Moe's speech in a similar way Sideshow Bob says "Hello, Bart." when they encounter.
Ms. Juniper drives a Nissan Cube in the scene where she, Melody, and Skinner leave Springfield. The Cube in the episode has some styling elements from both the second generation (Z11) Cube (which was only sold in Japan) and the third-generation (Z12) Cube (which was sold in North America, Europe, and Japan). It lacks the wrap-around rear window on the passenger side that is found on the real-life Cube beginning with the second-generation model.
When Grizzly Shaun talks to Mr. Burns in Moe's Tavern, Smithers has his normal suit on, however about two seconds later, he is wearing the suit he was when he entered the bar.
In the Banana Split Couch Gag, the ice cream contained whipped cream, chocolate syrup, and a cherry, and Santa’s Little Helper eats the entire ice cream, even though chocolate is poisonous to dogs.