The movie's plot revolves around Chuck and Larry posing as a gay couple for financial reasons, similar to Moe's adopting a gay image to enhance his business.
Like Smithers demanding that Moe kiss him in public to prove that he's gay, Chuck and Larry are asked to kiss in public (at a city council hearing) to prove that their relationship is real. Chuck and Larry's kiss is stopped short, however, when their boss arrives, "outs" them as being straight and exposes their scheme.
Homer, Lenny, and Carl and their late-shift counterparts (whom they fight) bear strong resemblances to each other, with only a few differences: Carl's counterpart wears glasses, Homer's has hair on the back of his head, and Lenny's has a different hair color and style.
Moe shows pictures of his bar after previous makeovers:
Whiskey A Moe-Moe (A makeover Moe never actually did, but a dream he painted)
Only two of those were failed, but The Nag and Weasel was a huge success has seen in the episode "Mommie Beerest".
Moe previously had the idea to give the bar a gay makeover in "Days of Wine and D'oh'ses". When Barney announced he was quitting drinking, Moe said, "To stay afloat, this bar's gonna have to go queer."
Several one-time gay characters from previous episodes reappear, including:
Dewey Largo, who has previously been hinted as being gay and closeted, reveals his sexuality in this episode as he runs off with his partner.
Smithers says "Hello, Moe." in Moe's speech in a similar way Sideshow Bob says "Hello, Bart." when they encounter.
Ms. Juniper drives a Nissan Cube in the scene where she, Melody, and Skinner leave Springfield. The Cube in the episode has some styling elements from both the second generation (Z11) Cube (which was only sold in Japan) and the third-generation (Z12) Cube (which was sold in North America, Europe, and Japan). It lacks the wrap-around rear window on the passenger side that is found on the real-life Cube beginning with the second-generation model.
When Grizzly Shaun talks to Mr. Burns in Moe's Tavern, Smithers has his normal suit on, however about two seconds later, he is wearing the suit he was when he entered the bar.