This episode marks the first appearance of The Seven Duffs. However, only Tipsy, Queasy, Surly and Remorseful are named in the episode.
The second Duff Gardens commercial shows Dolph, Jimbo, and Kearney getting arrested, yet they reappear near the end driving bumper cars into the sunset. In that shot it can also be seen that the "E" in "Duff Gardens" looks like an "F".
Lisa's underwear is exposed when she gets off the couch after Homer says that they were all going to Duff Gardens.
We learn Barney Gumble is a sperm donor and is a father of a huge amount of children.
On the magazine, "101 Frozen Pops", Professor Frink, Jacques, and Troy McClure (which is ironic, given that Selma would be with Troy McClure in "A Fish Called Selma") are featured, likely sperm donors like Barney.
One of the movies Homer and Marge watch while Homer is sick is The Erotic Adventures of Hercules, which was mentioned on "Mr. Plow" when Troy McClure hosted Carnival of the Stars.
This is one of the few episodes where Patty and Selma show no disgust of Homer, this was even shown when they raised their hands (along with Bart, Lisa and Maggie) for Homer to flash forward through Aunt Gladys' will reading.
Scenes in the episodes that were cut for time included the following:
After the scene where Homer hugs Patty and Selma and they subtly insult him within earshot, there's a short scene where Homer packs the stationwagon with their luggage. Bart complains, "Hey, Homer! This luggage is crushing me!" Homer retorts, "Hey, you don't hear Lisa complaining!" followed by a cut to show that Lisa isn't complaining... because she's so smushed up against the window that she can't even speak.
Bart and Lisa doing a variation of the counting game by counting the luggage falling off the car on the way to the funeral.
A scene where Marge, caring for a sick Homer, offers some rental tapes, Boxing's Greatest Weigh-Ins and Yentl. Homer asks what Yentl is and Marge tells him it's about a bookish young Jewish woman who poses as a man so she can go to rabbinical school. When Homer says Yentl sounds great, Marge concludes that he's delirious.
The Seven Duffs are a parody of the Seven Dwarves.
After hearing about the Sweathogs Selma said, "I checked, it isn't Horshack". This is a reference to Ron Palillo's character on the sitcom "Welcome Back, Kotter".
According to the DVD commentary, the end scene with Selma and Jub-Jub is a reference to the Murphy Brown episode, where Murphy's son Avery was born, which was a highly rated sitcom at the time this episode first aired.
After Bart Simpson pantsed the George Washington animatronic and caused the animatronic to turn around and glare at him ominously, Washington's pupils glowed red with a mechanical reverb being heard in the background, a reference to the Terminator franchise, in particular the titular character's characteristic red eye lenses.
In the first shot that shows Dolph, Jimbo, and Kearney stealing the bumper cars, Jimbo's bumper car is blue. In the next shot, Jimbo's bumper car changes to green.
While Lisa is intoxicated after drinking the "water" in Little Land of Duff, her skin tone alternates between a sickly pale greenish color and the normal yellow. When she is walking along with the parade, her skin is green, but when she starts dancing in front of the cars, her skin is yellow again. When she is returned to Selma by the security guards and deliriously declares herself the Lizard Queen, her skin is green again. When she gets back from Duff Gardens with Bart and Selma, her skin is yellow again, but when she says "Can't talk, coming down", her skin tone has settled on something in between the two tones.