The Twelfth Season was released August 18, 2009 in the US. It featured the episodes from the twelfth season. The spine features Matt Groening and Rainier Wolfcastle on one side and Agnes Skinner and Handsome Pete.
Release dates
| Region 1 | Region 2 | Region 4 |
| August 18, 2009 | September 28, 2009 | September 2, 2009 |
Disc Features
Disc 1
- Treehouse of Horror XI
- A Tale of Two Springfields
- Insane Clown Poppy
- Lisa the Tree Hugger
- Homer vs. Dignity
Disc 2
- The Computer Wore Menace Shoes
- The Great Money Caper
- Skinner's Sense of Snow
- HOMЯ
- Pokey Mom
- Worst Episode Ever
Disc 3
- Tennis the Menace
- Day of the Jackanapes
- New Kids on the Blecch
- Hungry, Hungry Homer
- Bye Bye Nerdie
- Simpson Safari
Disc 4
Bonus Features
- Optional commentaries for all 21 episodes
- Introduction from Matt Groening
- Deleted scenes with optional commentaries and option to watch during the episodes.
- Featurette: "Comic Book Guy: Best. Moments. Ever."
- Featurette: "The Global Fanfest"
- Sketch gallery
- Animation Showcases
- Illustrated commentaries
- Special language feature
- Commercials