We learn that Kodos is female; she is Kang's sister. However, this is contradicted in other episodes (probably because Kang either must have said "sister" by mistake or he is just confused of his gender).
The TV credits from the Season 2-early Season 20 opening sequence now has the "Created By/Developed By" TV credits in the same color and font as in the THOH end credits sequences.
The (now-defunct) Madison, WI-based ska band "I Voted For Kodos" took their name from Homer's line at the end of the "Citizen Kang" segment.
When Kang and Kodos turn into Bob Dole and Bill Clinton, the resulting light has been cut in TV airings to avoid seizures.
In this episode, the Gracie Films logo has an even higher pitched and shrill scream, which fans say that is slightly more scary than the original scream. It is possible that it is a little girl's scream, due to how higher it is compared to the woman's scream in most THOH episodes.
The Hunchback of Notre Dame: Hugo, representing Quasimodo, is living up in the attic.
Basket Case: Hugo wanting to be sewn back together.
The King and I: The segment title, "The Thing and I", is a parody of the film and play, "The King and I".
Twilight Zone: "The Genesis Tub" is inspired by the 1962 Twilight Zone episode "The Little People", where astronauts stumble upon a tiny civilization on an asteroid.
The Outer Limits: "The Genesis Tub" also closely resembles the two-part series premiere of the modern incarnation of "Outer Limits" titled "Sandkings" (parts I and II respectively) which first aired a year and a half prior to THOH VII. The main character "grows" his own mini-civilization which experiences rapid generational advances, and begins to build complex structures out of the sand. The main character becomes power-crazed with a "god complex".
Claw Game: The way Homer is abducted is reminiscent of the claw game (giant claw picking him up) and its difficulty (dropping him first time around).
Citizen Kane: The segment title, "Citizen Kang", is a parody of the 1941 film "Citizen Kane".
Scooby Doo: When Homer unmasks the presidential candidates, everyone has the same reaction as Scooby Doo and his gang whenever they rip off the masks of the villains.
Fish-Heads: Homer sings "Fish-Heads" by Barnes & Barnes while bringing a bucket full of fish heads to Hugo Simpson II.
The Exorcist: On the first disc of The Complete Fourteen Season, an alternate opening for the episode can be accessed via a Easter egg in the episode menu for Treehouse of Horror XIII The scene in question sees Reverend Lovejoy trying to exorcise Lisa (who is possessed) who acts in a similar manner to Regan MacNeil before her head spins around 360 Degrees and barfs the title on the camera.
Star Wars Episode IV: A New Hope: Kang commissioning the construction of a giant cannon to be fired at "a moon that [Earth] hadn't even heard of before" may have been a reference to the Death Star and its attempt at firing on Yavin 4.
In the first story, when Lisa asks whether she has two brothers the bottom of Lisa's neck is a single line that overlaps her necklace. This is likely a layering error where her neck is meant to be put behind her necklace but was accidentally put in front of it instead.
The lines on the glasses of the Lisa's World version of Professor Frink repeatedly disappear.
In the crowd cheering "Clinton", Apu has a black jacket instead of a green one, while Sanjay has yellow skin and a blue shirt.
In the same scene, Sanjay's green pants turn blue for a few frames.
In the shot after that when "Bob Dole" is about to speak, there is an empty space in the crowd between a few people that repeatedly is accidentally colored red.
The metal lines in the tanks containing the real candidates are in most shots at a height that covers their genitals. However, when the tanks are shown from the back, the metal lines appear significantly lower.